Inheritance of RAPD markers in the guppy fish, Poecilia reticulata.
Identifieur interne : 004036 ( Main/Exploration ); précédent : 004035; suivant : 004037Inheritance of RAPD markers in the guppy fish, Poecilia reticulata.
Auteurs : C L Foo [Singapour] ; K R Dinesh ; T M Lim ; W K Chan ; V P PhangSource :
- Zoological science [ 0289-0003 ] ; 1995.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN, Amorces ADN.
- génétique : ADN, Amorces ADN, Poecilia.
- médecine vétérinaire : Technique RAPD.
- ADN, Amorces ADN, Animaux, Données de séquences moléculaires, Femelle, Marqueurs génétiques, Mâle, Polymorphisme génétique, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, DNA (analysis), DNA (chemistry), DNA (genetics), DNA Primers (analysis), DNA Primers (chemistry), DNA Primers (genetics), Female, Genetic Markers, Male, Molecular Sequence Data, Poecilia (genetics), Polymorphism, Genetic, Random Amplified Polymorphic DNA Technique (veterinary).
- MESH :
- chemical , analysis : DNA, DNA Primers.
- chemical , chemistry : DNA, DNA Primers.
- chemical , genetics : DNA, DNA Primers.
- genetics : Poecilia.
- veterinary : Random Amplified Polymorphic DNA Technique.
- Animals, Base Sequence, Female, Genetic Markers, Male, Molecular Sequence Data, Polymorphism, Genetic.
Abstract
Random Amplified Polymorphic DNA (RAPD) fingerprinting offers a rapid and efficient method for generating a new series of DNA markers in fishes. Three oligonucleotide primers (two 10-mers and one 9-mer) and their paired combinations were found to generate different but reproducible RAPD fingerprints in the guppy. Of these, a 10-mer primer (designated S3D2) was used to detect DNA polymorphisms in two guppy varieties, Green Snakeskin (GSS) and 3/4Black (3/4B). High Genetic Similarity (SI) was found among individuals of the GSS and 3/4B varieties indicating low intra-variety genetic variability. The average SI values for the Green Snakeskin and 3/4Black varieties were 0.78 +/- 0.104 and 0.81 +/- 0.083, respectively. The average SI value between individuals of the GSS and 3/4B varieties was 0.66 +/- 0.066, indicating higher genetic variability between the two varieties. To study the inheritance of RAPD markers, single-pair crosses were set up between males of the GSS variety and females of the 3/4B variety. The S3D2 primer was used to generate RAPD fingerprints of the parents and their F1 offsprings. A total of 14 RAPD markers were scored from these crosses. Of these markers, eight (60.0%) of them were polymorphic. The RAPD markers were shown by the F1 to exhibit dominant Mendelian inheritance and could thus be used for subsequent genetic linkage mapping of the guppy.
DOI: 10.2108/zsj.12.535
PubMed: 8590830
Affiliations:
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Lim</name></author><author><name sortKey="Chan, W K" sort="Chan, W K" uniqKey="Chan W" first="W K" last="Chan">W K Chan</name></author><author><name sortKey="Phang, V P" sort="Phang, V P" uniqKey="Phang V" first="V P" last="Phang">V P Phang</name></author></analytic><series><title level="j">Zoological science</title><idno type="ISSN">0289-0003</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>DNA (analysis)</term><term>DNA (chemistry)</term><term>DNA (genetics)</term><term>DNA Primers (analysis)</term><term>DNA Primers (chemistry)</term><term>DNA Primers (genetics)</term><term>Female</term><term>Genetic Markers</term><term>Male</term><term>Molecular Sequence Data</term><term>Poecilia (genetics)</term><term>Polymorphism, Genetic</term><term>Random Amplified Polymorphic DNA Technique 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xml:lang="fr"><term>ADN</term><term>Amorces ADN</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Poecilia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term><term>Amorces ADN</term><term>Poecilia</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Technique RAPD</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Female</term><term>Genetic Markers</term><term>Male</term><term>Molecular Sequence Data</term><term>Polymorphism, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Amorces ADN</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Marqueurs génétiques</term><term>Mâle</term><term>Polymorphisme génétique</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Random Amplified Polymorphic DNA (RAPD) fingerprinting offers a rapid and efficient method for generating a new series of DNA markers in fishes. Three oligonucleotide primers (two 10-mers and one 9-mer) and their paired combinations were found to generate different but reproducible RAPD fingerprints in the guppy. Of these, a 10-mer primer (designated S3D2) was used to detect DNA polymorphisms in two guppy varieties, Green Snakeskin (GSS) and 3/4Black (3/4B). High Genetic Similarity (SI) was found among individuals of the GSS and 3/4B varieties indicating low intra-variety genetic variability. The average SI values for the Green Snakeskin and 3/4Black varieties were 0.78 +/- 0.104 and 0.81 +/- 0.083, respectively. The average SI value between individuals of the GSS and 3/4B varieties was 0.66 +/- 0.066, indicating higher genetic variability between the two varieties. To study the inheritance of RAPD markers, single-pair crosses were set up between males of the GSS variety and females of the 3/4B variety. The S3D2 primer was used to generate RAPD fingerprints of the parents and their F1 offsprings. A total of 14 RAPD markers were scored from these crosses. Of these markers, eight (60.0%) of them were polymorphic. The RAPD markers were shown by the F1 to exhibit dominant Mendelian inheritance and could thus be used for subsequent genetic linkage mapping of the guppy.</div></front></TEI><affiliations><list><country><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><noCountry><name sortKey="Chan, W K" sort="Chan, W K" uniqKey="Chan W" first="W K" last="Chan">W K Chan</name><name sortKey="Dinesh, K R" sort="Dinesh, K R" uniqKey="Dinesh K" first="K R" last="Dinesh">K R Dinesh</name><name sortKey="Lim, T M" sort="Lim, T M" uniqKey="Lim T" first="T M" last="Lim">T M Lim</name><name sortKey="Phang, V P" sort="Phang, V P" uniqKey="Phang V" first="V P" last="Phang">V P Phang</name></noCountry><country name="Singapour"><noRegion><name sortKey="Foo, C L" sort="Foo, C L" uniqKey="Foo C" first="C L" last="Foo">C L Foo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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